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How to make agar agar?

How to make an nutrient agar?

  • nutrient agar

  • Answer:

    Nutrient agar is a basic non-selective medium used for growing bacteria generally. meat extract 3g peptone (bactopeptone) 10g NaCl 5g agar 15g distilled water 1L Sterilization by autoclaving 121 °C, 20 min.

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Yeast extract 4.0 casein hydrolysate 5.0 D(+)glucose 50.0 potassium dihydrogen phosphate 0.55 potassium chloride 0.425 calcium chloride 0.125 magnesium sulfate 0.125 Iron(III) chloride 0.0025 manganese sulfate 0.0025 bromocresol green 0.022 agar-agar 17.0 This is for g/liter..though composition many vary as these days many things from above are not added so that medium dosent get selective.

Viku

Depends what you want it for, yeast, bacteria, some particular type of bacteria. And weather you want to place some sort of selection on what you're growing, e.g. antibiotics or nutritional selection.

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