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How to find molecular mass?

How to find the centrifugation time, speed to centrifuge out sample of known mass / molecular weight / size?

  • Is there any mathematical equation to find the centrifugation time , speed (rpm) for any sample of known mass,size and molecular weight ? I have a protein loaded to my porous nanoparticle. Now I have to study the loading stabilty in course of time. My idea is to shake it and measure the protein released. But what centrifugation rpm and time i have to use to seperate just the protein released from the Nanoparticle-protein conjugate ? I have checked in papers but couldn't find such experiments.. Could any one please help me out to find any equation for calculating these stuffs. It will be great help indeed =) Thank You very much =) I can give more details if needed =)

  • Answer:

    It all depends on the masses, shapes, viscosity, the rotor radius, and possible rotor velocities. The centrifugation should probably proceed very fast not to disturb any equilibria to much. You will not be able to sediment your protein very fast, except in a ultracentrifuge. So the released protein will be in the supernatant. Now it will depend on the size, and mass of the nanoparticle, in which time, and with what gravitational field, you can sediment this protein loaded particle. If you know the Svedberg constant s of the particle, you can find the angular speed w needed at a given rotor radius R , and a expected sedimentation velocity by s = v/(w^2*R). If the s - value does not change too much between free and loaded particles. But I guess you could also check it out by running a few nanoparticle samples at different velocities to see under what condition you can sediment them. OG

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It all depends on the masses, shapes, viscosity, the rotor radius, and possible rotor velocities. The centrifugation should probably proceed very fast not to disturb any equilibria to much. You will not be able to sediment your protein very fast, except in a ultracentrifuge. So the released protein will be in the supernatant. Now it will depend on the size, and mass of the nanoparticle, in which time, and with what gravitational field, you can sediment this protein loaded particle. If you know the Svedberg constant s of the particle, you can find the angular speed w needed at a given rotor radius R , and a expected sedimentation velocity by s = v/(w^2*R). If the s - value does not change too much between free and loaded particles. But I guess you could also check it out by running a few nanoparticle samples at different velocities to see under what condition you can sediment them. OG

Ossi G

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