How to make LB agar using LB broth base and plain, granulated agar?
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Hello folks. I, being a fresher in molecular biology, accidentally made agar plates out of plain, granulated agar. Post plating and incubation, there was no growth. The bacteria and ...show more
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Answer:
If you have the 1X LB broth, (i.e. tryptone, yeast extract & NaCl), then just add your agar powder at a concentration of 15 g/liter, for just a regular agar plate. Make sure you dissolve the agar after you've added it--when you autoclave it, it will dissolve.
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