What is cloning?

What is a primary difference between PCR and traditional cloning procedures such as those used to clone the hu?

• What is a primary difference between PCR and traditional cloning procedures such as those used to clone the human growth hormone gene? a PCR uses plasmid vectors, whereas traditional cloning uses bacteria. b PCR eliminates the need for restriction enzymes, plasmid vectors, and transformed cells. c PCR is more time-consuming, but the purity of the obtained DNA clone is much higher than in traditional cloning. d PCR and traditional cloning make use of different types of bacteria. Why might retroviral vectors for gene therapy increase the patient's risk of developing cancer? a They might introduce proteins from the virus. b They might integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integration. c They might not express the genes that were introduced into a patient's cells. d They might not integrate their recombinant DNA into the patient's genome.

• Answer:

  b b