A gene has the base sequence that starts with GAC TGA CGA TTA. a) What would be the complementary DNA stra?
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3. A gene has the base sequence that starts with GAC TGA CGA TTA. a) What would be the complementary DNA strand formed from this DNA? (3 points) b) What would be the mRNA base sequence formed from this DNA sequence? (3 points) c) Use the codon table below to find the first three amino acids in the protein formed from this gene. (3 points) d) The gene has a mutation and is changed to the sequence below. GAC GGA CGA TTA What kind of mutation is this? (2 points) e) What is the new mRNA strand produced by this mutated gene? (3 points) f) Does this mutation affect the protein produced? Explain. g) What process creates mRNA? (2 points
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Answer:
3a) since A binds to T and G binds to C the complimentary strand is CTG ACT GCT AAT 3b) since A binds to T, G binds to C. but when a T is needed on the mRNA strand a U is used instead of a T. Do not use 3a strand, use the original question strand. CUG ACU GCU AAU 3c) Make the mRNA strand a tRNA strand by base pairing again, and again a U is used not a T. GAC UGA CGA UUA So now match GAC, UGA, CGA with amino acids on the codon table 3d) T and G have swapped over in the 2nd triplet code. This is a substitution gene mutation. 3e) CUG CCU GCU AAU 3f) convert the CCU into tRNA by base paring again. CCU -> GGA Now look at the amino acid table and look at what amino acid GGA codes for. Ask yourself, is this amino acid the same amino acid as UGA? if it is different then the mutation does affect the protein produced 3g) transcription
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